Molecular Cloning Tools
Designers and checkers for the cloning methods you actually use at the bench.
Gibson Assembly Calculator
Check homology arms and assemble multiple DNA fragments into one sequence.
Open tool →Golden Gate Assembly Tool
Check 4 nt overhang compatibility and assemble Type IIs parts in one pot.
Open tool →In-Fusion Cloning Calculator
Design 15 bp homology arms and assemble fragments by homologous recombination.
Open tool →Gateway Cloning Calculator
Locate attB sites on vector and insert and generate the expression clone.
Open tool →SLiCE Cloning Calculator
Plan Seamless Ligation Cloning Extract reactions with 25 bp homology arms.
Open tool →TA & TOPO Cloning Calculator
Simulate TA and TOPO cloning of a PCR product into a linear vector.
Open tool →Site-Directed Mutagenesis Tool
Design primers for point mutations, insertions and deletions in one step.
Open tool →