Plasmid Designer

Upload a GenBank or FASTA file to draw a circular plasmid map, annotate restriction enzyme sites, and export the map as PNG or SVG.

Upload File

No plasmid loaded

Upload a .gb / .fasta file or load the sample plasmid to draw a circular map with restriction enzyme sites.

Description

A browser-based plasmid designer. Parse GenBank or FASTA input, render a circular plasmid map with annotated features, detect restriction enzyme cut sites from a built-in database of common enzymes, and click any site to see the cut position and expected fragment sizes. Export the map as PNG or SVG for lab notebooks or publications.

How to use

1) Click Upload File to load a GenBank (.gb) or FASTA (.fasta) file, or load the pUC19 sample. 2) Tick the restriction enzymes you want to test in the left panel. 3) The circular map shows features (colored arcs) and enzyme cut sites (red ticks). 4) Click a red tick (or a row in the site list) to view the cut position and fragment sizes. 5) Click PNG or SVG to export the map.