Primer Design & QC Tools

Design primer pairs from a template, check them for hairpins and dimers, and work out what volume your tube should be resuspended to.

In-Silico PCR Tool

Predict every amplicon a primer pair can produce on your template.

Fuse two fragments by SOE PCR and check the overlap before you order primers.

Design PCR primer pairs from a template and a target region, with Tm, GC content and product size for each pair.

Calculate primer melting temperature (Tm) using the Wallace rule and salt-adjusted formula, with GC content and length.

Check a primer for hairpins and dimers, and scan a template for off-target binding sites with mismatch tolerance.

Calculate oligo molecular weight, GC content and extinction coefficient, plus the water volume that gives a 100 µM stock.

PCR helper calculator — estimate annealing temperature, extension time, product mass, and amplification fold from cycle count.