Primer Design & QC Tools
Design primer pairs from a template, check them for hairpins and dimers, and work out what volume your tube should be resuspended to.
In-Silico PCR Tool
Predict every amplicon a primer pair can produce on your template.
Open tool →Overlap Extension PCR Tool
Fuse two fragments by SOE PCR and check the overlap before you order primers.
Open tool →Primer Design Tool
Design PCR primer pairs from a template and a target region, with Tm, GC content and product size for each pair.
Open tool →Primer Tm Calculator
Calculate primer melting temperature (Tm) using the Wallace rule and salt-adjusted formula, with GC content and length.
Open tool →Primer Checker
Check a primer for hairpins and dimers, and scan a template for off-target binding sites with mismatch tolerance.
Open tool →Oligo Calculator
Calculate oligo molecular weight, GC content and extinction coefficient, plus the water volume that gives a 100 µM stock.
Open tool →PCR Calculator
PCR helper calculator — estimate annealing temperature, extension time, product mass, and amplification fold from cycle count.
Open tool →