DNA Analysis Tools
Free online DNA sequence analysis tools — reverse complement, GC content, base counter, sequence cleaner, transcription, ORF finder, primer Tm, PCR calculator, and agarose gel simulation.
DNA Reverse Complement
Compute the reverse complement of a DNA sequence (A↔T, C↔G) instantly.
Open tool →GC Content Calculator
Calculate the GC content percentage and base composition of a DNA sequence.
Open tool →DNA Base Counter
Count the occurrence of each nucleotide (A, T, C, G) in a DNA sequence.
Open tool →DNA Sequence Cleaner
Clean a DNA sequence by removing whitespace, numbers, and non-standard characters.
Open tool →DNA to RNA Transcription
Transcribe a DNA sequence into RNA by replacing thymine with uracil (T→U).
Open tool →ORF Finder
Find open reading frames (ORFs) in a DNA sequence across all 6 reading frames, from start (ATG) to stop codon.
Open tool →Agarose Gel Calculator
Recommend agarose gel percentage by DNA size and calculate agarose powder and buffer volumes for gel preparation.
Open tool →GC Content Distribution
Plot GC percentage along a DNA sequence with a sliding window to spot GC-rich and AT-rich regions.
Open tool →Local Alignment + Dot Plot
Compare two DNA sequences with a dot plot and Smith-Waterman local alignment, showing score and identity.
Open tool →Restriction Analysis
Find restriction sites in a sequence and simulate single or double digests with fragment sizes.
Open tool →Restriction Enzyme Database
Search restriction enzymes by recognition site, overhang, buffer, methylation sensitivity and supplier.
Open tool →CRISPR gRNA Design
Find SpCas9 target sites with PAM, GC content and an on-target quality score.
Open tool →Codon Optimization
Reverse-translate a protein into DNA using host codon usage, or optimize an existing CDS.
Open tool →Synonymous Mutation Design
Add or remove restriction sites without changing the encoded protein sequence.
Open tool →Chromatogram Viewer (.ab1)
Open Sanger .ab1 trace files in your browser and read called bases with quality values.
Open tool →