Cell Culture Calculator

Cell counting, seeding, split ratio, and viability calculations.

Calculation Mode

Total cells counted

Number of squares counted

Dilution factor (e.g. 2 for 1:1 trypan blue)

Total sample volume (mL, optional)

Formula: cells/mL = (count ÷ squares) × dilution × 10⁴

Result

Enter the required values to calculate.

Description

Common cell culture calculations: hemocytometer cell counting, seeding volume for a target cell number, split ratio for passaging, and viability from Trypan blue exclusion.

How to use

Select a mode. For cell counting, enter the total cells counted and the number of squares. For seeding, enter the stock density and target cell number. For split ratio, enter current and desired densities. For viability, enter live and dead cell counts.