Assay & Quantification Tools

Fit calibration and dose-response curves, convert enzyme activity units, and work out qPCR efficiency, DNA copy number or protein concentration.

Dose-Response Curve Fit (IC50)

Fit a 4-parameter logistic curve to dose-response data and solve IC50 or EC50.

Open tool →

qPCR Standard Curve Calculator

Fit a qPCR standard curve from your Cq values and read off amplification efficiency, slope, R² and ΔCq — then back-calculate an unknown sample's template quantity.

Open tool →

DNA Copy Number Calculator

Convert DNA mass to molecule copy number (and back) from template length, with a quick-reference table for amplicons, plasmids, E. coli and human genome templates.

Open tool →

Protein Assay Calculator (BCA/Bradford)

Build a BCA or Bradford standard curve, interpolate sample absorbance to protein concentration, and correct for the dilution factor.

Open tool →

Enzyme Activity Unit Converter

Convert between U, mU, nkat, µkat and katal, or compute activity from an absorbance rate using extinction coefficient, path length and protein concentration.

Open tool →

Statistics Calculator (t-test & ANOVA)

Summarise data with mean, SD, SEM and 95% CI, or run Welch's t-test, a paired t-test and one-way ANOVA with exact p values.

Open tool →

Nucleic Acid Quantification (A260/A280)

Convert A260, A280 and A230 readings into DNA or RNA concentration, total yield, purity ratios and pmol/µL.

Open tool →