Serial Dilution Calculator

Compute concentration at each step of a serial dilution.

Dilution Parameters

Initial concentration

Dilution factor

Factor of 2 means each step is half the concentration (1:2). Factor of 10 means 1:10.

Number of steps

Volume per step (mL, optional)

If provided, calculates transfer and diluent volumes per step.

Formula: Cₙ = C₀ / factorⁿ

Dilution Table

Enter initial concentration, dilution factor, and number of steps.

Description

A serial dilution is a stepwise dilution of a stock solution, where each step dilutes the previous one by a fixed factor. It is commonly used to prepare standard curves, dose-response series, and microbial counts.

How to use

Enter the initial concentration, dilution factor (e.g. 2 for a 2-fold dilution), and the number of steps. The concentration at each step is computed instantly. Optionally enter a per-step volume to also see the transfer volume and diluent volume per step.